Dye for Mindray 5-Part Hematology Analyzers kɔnɔ, polimetini ni asidi nukiliki kalali kɛrɛnkɛrɛnnenw bɛ sɔrɔ minnu bɛ siri kɛrɛnkɛrɛnnenya la selilɛri ADN ni ARN la. O fɛnw bɛ joli kuru finmanw, reticulocytes ani platelets taamasiyɛn kɛ flow cell kɔnɔ, o bɛ kɛ sababu ye ka scattergramme faranfasi yɔrɔ caman na semiconducteur laser yeelen jɛnsɛnni fɛ ani fluorescence detection fɛ.

Hematology Stain / Dye Reagents ye mun ye?

Jolilabanaw ka nɔgɔ/kalali fura minnu bɛ kɛ ka ɲɛsin otomatiki sɛgɛsɛgɛlikɛlanw ma, olu ye kemikɛli furaw ye minnu kɔnɔ, fluorophores organiques, surfactants ani osmotiques stabilisateurs bɛ sɔrɔ. Nin kalali jilamaw tɛ i n’a fɔ bololabaarakɛminɛn minnu bɛ kɛ ni joli-sira-funu-minɛnw ye, olu bɛ baara kɛ ni jibɔlan otomatiki jibɔlan dagalenw kɔnɔ.

Ni a pikiri kɛra detekni kɛcogo yɔrɔ la, kalali bɛ don selilɛriw kɔnɔ minnu labɛnna ni lise sɛgɛsɛgɛlikɛlan kɛrɛnkɛrɛnnenw ye, ka siri stoikiometri la ni asidi nukiliki ye selilu kɔnɔ. Ni u bilala laser-fiɲɛ semikɔndukiri la flow cell kɔnɔ, fluorophores sirilenw bɛ yeelen bɔ kɛrɛfɛ min bɛ bɛn selilɛri nukilisi asidi hakɛ ma.

Jɔrɔ min bɛ selilɛriw danfara / dɔnni na

Fluorescent dye reagents (Fluorescent dye reagents) bɛ kɛ sababu ye ka joli kuru gɛlɛnw danfara ɲɛ ye:
Asidi nukiliki taamasiɲɛw:Fluorophores bɛ don selilɛri kɔnɔ ARN ni ADN sɛrɛkiliw kɔnɔ. Bugun minnu farikolojɔli baara ka bon walima minnu ma kɔgɔ, olu bɛ kalali hakɛ caman minɛ, o bɛ taamasiɲɛ barikamaw di minnu bɛ yeelen bɔ.
Scattergram (Scattergram) sɛnɛcogo:Kɛrɛfɛla jɛnsɛnni yeelen ni kɛrɛfɛla fluorescence fanga faralen ɲɔgɔn kan, o bɛ scattergrams multi-dimensionnels dilan.
Cellules Sɛgɛsɛgɛli min tɛ cogo la:Fluorescent emission profiles danfaralenw bɛ lymphocytes atypicals, granulocytes kɔgɔbaliw ani joli kuru bilenmanw nucléaires bɔ joli kuru finman kɔgɔlenw na.

Kɔlɔsili siraw

Kanal min bɛ detekti	Kanal Tɔgɔ / Laɲini	Biologique Function & Sumanikɛcogo
WNR Kanal ye	Cellule blanc nucléaire & Kanal RBC	A bɛ WBC hakɛ bɛɛ lajɛlen suman ani ka NRBC jate. Basofiliw bɛ danfara don farikolo jɛnsɛnni fɛ ka sɔrɔ NRBC nukilisi asidiw bɛ taamasiyɛn kɛ ni fluorescent ye.
WDF Kanal ye	Kanal Différentiel Cellule Blanc	A bɛ danfara don Neutrophils, Lymphocytes, Monocytes ani Eosinophiles (Eosinophiles) ni ɲɔgɔn cɛ. A bɛ Granulocytes kɔgɔbaliw suman ni asidi nukiliki fluorescent kalali sirili fanga ye.
WPC Kanal ye	Kanal Precurseur de Cellule Blanc	Sɛgɛsɛgɛliw bɛ kɛ ka ɲɛsin bangebagaw ma, farikolojidɛsɛbanakisɛw, ani blasiw. A bɛ baara kɛ ni membrane perméabilité reaction kɛrɛnkɛrɛnnen ye min faralen don ɲɔgɔn kan ni kalali tali ye walasa ka blast cellules danfara.
RET Kanal ye	Kanal min bɛ wele ko reticulocyte	A bɛ Reticulocyte hakɛ, jate dafalen, Immature Reticulocyte Fraction, ani Red Blood Cell equivalent parameters suman ARN fluorescent staining fɛ.
PLT Kanal ye	Kanal Optique de plaquettes	A bɛ joli segin jate kɛ ni ɲɛkisɛw ye ni kalali fluorescent labeling ye joli segin furakisɛw ARN kan, ka fɛnw bali ka bɔ RBC mikrositikiw walima nɔgɔw la.

Analyzer Series min bɛ bɛn ɲɔgɔn ma

Laser Hématologie Serie automatique 5-Part:A dabɔra jisigiyɔrɔw kama minnu bɛ baara kɛ ni semikɔnduktɛri laser fluorescence flow cytometry ye.

High-Throughput Modular Hematology Baarakɛyɔrɔw:A bɛ se ka kɛ ni otomatiki cap-piercing samplers ye ani doni tali basigilenw ye.

Joli-yɔrɔ-ko jɛlen-Slide Maker Systems:A sɛgɛsɛgɛra ka ɲɛsin reagent directe inline delivery systems ma ni signal drift walima pressure variation tɛ.

Fɛn dilannenw ka ɲɛfɔli

Paramɛtiri	Tekniki sariya / Donanw
Furakɛcogo suguya	Jilama min bɛ kɛ ni kalali ye min bɛ kɛ ni fluorescent ye
Fluorophore Base min bɛ baara kɛ	Polimetini / Asidi nukiliki kalali min bɛ kɛ ni fluorescent ye
Excitation (ɲɛnabɔli) Wavelength (Wavelength).	633 nm – 635 nm (Laser semiconducteur bilenman) .
pH hakɛ (degere 25 ) .	7.00 – 8.50
Ɲɛgɛnɛsiraw (degere 25 ) .	1,20 – 2,50 mS/cm
Density (20 degɛrɛ ) .	1,005 – 1,020 g/cm3 ye
Da wuli-Viyali sabatili	Tile 60 (degere 2 – degere 30 dibi marali la) .
Shelf Life Dabɔbali	Kalo 18 kɔnɔ
A maracogo funteni	degere 2 – degere 30 (Aw bɛ aw tanga yeelen ni nɛnɛ ma) .
Filtration particulaire (Filtration particulaire) ye	A bɛ filɛri kɛ 0,2 μm membrane fɛ

Aplikasi & Reagent Baarakɛcogo

WNR ka danfara sɛgɛsɛgɛli:A pikiri bɛ kɛ WNR farikoloɲɛnajɛyɔrɔ la walasa ka joli kuru bilenmanw nukililenw ni joli kuru finmanw nukiliw taamasiɲɛ kɛ sitoplasma wulilen kɔfɛ.

WDF 5-Part WBC ka danfara:A bɛ baara kɛ ni WBC jamanaden fitininw ye WDF kanal kɔnɔ, ka fluorescence kulu danfaralenw dilan ka ɲɛsin lanposotiw, monositɛriw, neutrophils ani eosinophiles ma.

Precursor & Blast Cell ka darapo:A dabɔra walasa ka don lipid membranes caman caman cili kɔnɔ ka sugandili kɛ WPC kanal kɔnɔ, o bɛ a to ka se ka selilu hakɛ kɔrɔbaliw dɔn.

ARN retikulositɛri hakɛ jateminɛ:A bɛ siri sitoplasma ribosomali ARN la retikulocytes kɔnɔ, o bɛ sira di reticulocytes kɔgɔli taabolo jatebɔ ma.

Jate min bɛ kɛ ni plaquettes optique ye:A bɛ joli segin furakisɛw kɔnɔna ARN yɔrɔw nɔgɔ walasa ka ɲɛnayeli jatew lawuli joli segin furakisɛw kɛnɛyaso cogoyaw la minnu ka dɔgɔn-.

Foroko / Ladonni

Minɛn	Kɛrɛnkɛrɛnnenya la
Forobaciyɛn min bɛ kɛ ka kɛɲɛ ni sariya ye	mililitiri 12 x buteli 1 / kɛsu, mililitiri 42 x buteli 1 / kɛsu
Butɛri Materiel	HDPE buteli minnu bɛ ni fluor ye amber ye minnu bɛ yeelen-kɔlɔsi
A maracogo funteni	2 degere fo 30 degere
Transport (Transport) wajibiyalenw	Aw bɛ aw tanga nɛnɛ ma ani tile yeelen tilennen ma

Jolilabanaw (Reactifs de hématologie) minnu bɛ tali kɛ ɲɔgɔn na

Diluent Solution for 5-Part Hematology Analyzers (Hématologie analyseurs) minnu bɛ kɛ ni yɔrɔ 5 ye:Tampon isotonique conducteur solution min bɛ joli tacogo dilan ani ka kɔkan mankanw bali.

Lyse Reagent for 5-Part Hematology Analyzers (Sɛgɛsɛgɛlikɛlan minnu bɛ jolilabanaw lajɛ minnu bɛ kɛ yɔrɔ 5 la:Surfactant-based lysing solution walasa ka eritoromisini membrane membrane sugandilen wuli.

Saniya fura min bɛ kɛ ni fɛn caman ye:Ji saniyalan min bɛ kɛ ka ji layini ladon otomatiki la ani ka farikolojɔli dumuniw bɔ.

Sonda Saniyalan:Alkaline hypochlorite sonda kolanfɛn min bɛ kɛ ka sample sonda deprotéinisation kɛ.

Ɲininkaliw

J: Kɔlɔsilikɛlan min bɛ kɛ ni fluorescent otomatiki ye, o ni joli-sira-funu-nɔgɔlan kɔrɔlenw tɛ kelen ye cogo di?

A: Laadalata nɔgɔw bɛ a ɲini ka u ja ni bolo ye gilasi slayidiw kan walasa ka u jateminɛ ni ɲɛ ye mikroskɔpu fɛ. Analyzer dye reagents automatiques ye kalali fluorescent jilamaw ye minnu dabɔra inline reaction chambers kama. U bɛ siri selilɛriw ka ARN/ADN la jilama kɔnɔ, ka yeelen taamasiɲɛw bɔ minnu bɛ sɔrɔ laser flow cytometry optics fɛ.

J: Yala yeelen bɔli bɛ nɔ bila kalali baara la don o don baara kɛtuma na wa?

A: Ɔwɔ. Fluorophores actifs bɛ tiɲɛ ni u bilara yeelen tilennen na walima yeelen UV lamini na waati jan kɔnɔ. Reagents bɛ pake amber minɛnw kɔnɔ minnu bɛ yeelen-kɔlɔsili kɛ, wa u ka kan ka to reagents yɔrɔ datugulen kɔnɔ baara kɛtuma na.

J: Fɛn marayɔrɔ funteni jumɛn de ka kan ka kɛ bolifɛnw ni fɛnmarayɔrɔw la?

A: Aw bɛ reagents mara degere 2 ni degere 30 cɛ . Ni a marali bɛ degere 0 duguma, o bɛ kɛ sababu ye ka fluorophore (fluorophore) sanji bɔ. Ni funteni bɛ tɛmɛ degere 35 kan, o bɛ fɛnɲɛnamafagalanw tiɲɛni teliya.

J: Lot-to{1}}lot ɲɔgɔndan bɛ nɔ bila scattergram jaabiw la cogo di?

A: Yɛlɛma minnu bɛ kɛ kalali hakɛ la, olu bɛ na ni scattergram cluster shifts ye, o bɛ na ni gating filiw ye. Fɛn dilanni yɔrɔ kelen-kelen bɛɛ bɛ kɛ ni fluorophore saniya sɛgɛsɛgɛli ye, ka spectrofluorometric sɛgɛsɛgɛli kɛ, ani ka matrix matching kɛ walasa ka scattergram positioning basigilen mara.

J: Pakew dilanni jumɛn bɛ kɛ ka ɲɛsin jilama jɛɲɔgɔnya ma?

A: Kalafɛnw bɛ dilan HDPE buteliw kɔnɔ minnu datugulen don ni fluor ye, ni u dabɔlanw ye minnu dabɔra ji minɛcogo tilennenw kama, o bɛ wuli ni nɔgɔli bali.

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