Sugunɛbilenni sɛgɛsɛgɛlikɛlan ye minɛn ye min nafa ka bon bi furakɛli sɛgɛsɛgɛliw la. A bɛ kɛ ka caya kɛnɛyaso sugunɛ sɛgɛsɛgɛli la walasa ka dɔgɔtɔrɔw dɛmɛ ka sugunɛbaralabanaw, sugunɛbanaw ani kɛnɛyako gɛlɛya wɛrɛw sɛgɛsɛgɛ joona. I n’a fɔ fɛnba min bɛ se ka dun sɛgɛsɛgɛli kɛcogo la, sugunɛbilenni sɛgɛsɛgɛlikɛlanw kɛcogo bɛ nɔ bila sɛgɛsɛgɛli jaabiw tiɲɛni n’u dannaya la k’a ɲɛsin u yɛrɛ ma. A yɔrɔ kolomaw faamuyali bɛna dɔgɔtɔrɔsow ni laboratuwaraw dɛmɛ ka furaw sugandi ka ɲɛ ani ka baara kɛ ni u ye minnu bɛ tali kɛ ɲɔgɔn na.
A ka c’a la, sugunɛbilenni sɛgɛsɛgɛlikɛlanw yɔrɔ kolomaw ye kalalifɛnw, tamponw ani surɔfɛnw ye. Kɔlɔsili ye fɛnɲɛnamafagalanw yɔrɔ koloma dɔ ye, minnu bɛ kɛ ka seliluw, fɛnw, kristalɛw ani fɛn wɛrɛw danfara bonya sugunɛ na, o b’a to u dɔnni ka nɔgɔn mikroskɔpu fɛ. Kala minnu ka teli ka kɛ olu ye eosin, methylene blue, a ɲɔgɔnnaw ye O kalali ninnu bɛ se ka siri kɛrɛnkɛrɛnnenya la sugunɛ kɔnɔfɛn suguya caman na walasa ka sɛgɛsɛgɛli kɛcogo ni a kɛrɛnkɛrɛnnenya ɲɛ.
Tampon jɔyɔrɔ ka bon pH hakɛ sabatili la reagent kɔnɔ, ka a to sɛgɛsɛgɛli kɛyɔrɔ bɛ pH hakɛ bɛnnen kɔnɔ. Sugunɛ tacogo pH hakɛ bɛ se ka yɛlɛma ka da danfara kelen-kelen bɛɛ kan walima fɛn dɔw fɛ i n’a fɔ dumunikɛcogo, wa tamponw bɛ se ka o fɛn caman wuli ka bɔ u nɔ na, ka kalalifɛnw ni sugunɛ yɔrɔw ka farikoloɲɛnajɛ sabatili sabati, o la, sɛgɛsɛgɛli jaabiw seginni ni u tiɲɛni bɛ ɲɛ.
Surfactants bɛ kɛ ka fɛnɲɛnamafagalanw jɛnsɛn sugunɛ na, ka selilu walima tubabukanw fara ɲɔgɔn kan, ani ka a lajɛ ni sɛgɛsɛgɛli kɛcogo bɛ kɛ kelen ye. O nafa ka bon kosɛbɛ sugunɛbilenni sɛgɛsɛgɛlikɛlanw na minnu bɛ kɛ otomatiki la, barisa a ka c’a la, minɛn in bɛ tali kɛ ɲɛnayeli walima jibɔ fɛɛrɛw la walasa ka a dɔn, wa, sɛgɛsɛgɛli kɛlenw ka kelenya bɛ nɔ bila sɛgɛsɛgɛli jaabiw la k’a ɲɛsin u yɛrɛ ma.
Ka fara o kan, sugunɛbilenni sɛgɛsɛgɛlikɛlan dɔw fana bɛ se ka fɛnɲɛnamafagalanw walima sabatilifɛnw fara u kan walasa ka fɛnɲɛnamafagalanw marali waati janya ani ka dɔ bɔ u tiɲɛni walima u tiɲɛni na min bɛ sɔrɔ u maracogo caman yeli fɛ. Nin farali ninnu bɛ se ka kɛ sababu ye ka fɛnɲɛnamafagalanw sabati u tali ni u marali waati, o la, furakɛlitɔnw magow ɲɛ fɛnɲɛnamafagalanw sɔrɔli la waati jan kɔnɔ.
Sugunɛbilenni sɛgɛsɛgɛlikɛlanw yɔrɔ kolomaw faamuyali bɛ se ka laboratuwari baarakɛlaw dɛmɛ u k’u ka baarakɛcogo faamuya ka ɲɛ ani ka u sinsin baarakɛcogo jiralan jɔnjɔnw kan ni u bɛ fɛnɲɛnamafagalanw sugandi. Ni dɔnni fɛɛrɛw bɛ ka taa ɲɛ, sugunɛbilenni sɛgɛsɛgɛlikɛlanw dilanni bɛ se ka ɲɛ ka taa a fɛ don nataw la walasa ka dɔnni kɛcogo ɲuman n’a tiɲɛni ɲɛ, o bɛ dɛmɛ don min bɛ se ka da a kan kosɛbɛ kɛnɛyaso sɛgɛsɛgɛli la.